3zvu

X-ray diffraction
2.1Å resolution

Structure of the PYR1 His60Pro mutant in complex with the HAB1 phosphatase and Abscisic acid

Released:

Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Abscisic acid receptor PYR1 Chain: A
Molecule details ›
Chain: A
Length: 193 amino acids
Theoretical weight: 21.66 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O49686 (Residues: 3-191; Coverage: 99%)
Gene names: ABIP6, At4g17870, PYR1, RCAR11, T6K21.50
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Structure domains: Alpha-D-Glucose-1,6-Bisphosphate; Chain A, domain 4
Protein phosphatase 2C 16 Chain: B
Molecule details ›
Chain: B
Length: 337 amino acids
Theoretical weight: 37.35 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CAJ0 (Residues: 178-511; Coverage: 68%)
Gene names: At1g72770, F28P22.4, HAB1, P2C-HA
Sequence domains: Protein phosphatase 2C
Structure domains: PPM-type phosphatase domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P212121
Unit cell:
a: 45.48Å b: 66.05Å c: 172.39Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.18 0.236
Expression system: Escherichia coli BL21(DE3)