› 3zux

X-ray diffraction
2.2Å resolution

Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.

Released:
DOI: 10.2210/pdb3zux/pdb
PDB entry 3zux coloured by chain, front view.
PDB entry 3zux coloured by chain, side view.
PDB entry 3zux coloured by chain, top view.
Source organism: Neisseria meningitidis
Primary publication:
Crystal structure of a bacterial homologue of the bile acid sodium symporter ASBT.
Hu NJ, Iwata S, Cameron AD, Drew D
Nature 478 408-11 (2011)
PMID: 21976025

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-217106 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transporter Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 34.77 KDa
Source organism: Neisseria meningitidis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9K0A9 (Residues: 1-315; Coverage: 100%)
Gene name: NMB0705
Sequence domains: Sodium Bile acid symporter family
Structure domains: Na+/H+ antiporter like fold

Ligands and Environments

5 bound ligands:
Ligand NA 2 x NA
Ligand HG 1 x HG
Ligand TCH 2 x TCH
Ligand LDA 5 x LDA
Ligand PTY 2 x PTY
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P4122
Unit cell:
a: 73.33Å b: 73.33Å c: 163.52Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.229
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

38 review citations

The ABCs of membrane transporters in health and disease (SLC series): introduction.
Hediger et al. (2013)
37 more

17 mentions without citation

The solute carrier family 10 (SLC10): beyond bile acid transport.
Claro da Silva et al. (2013)
16 more