› 3zuv

X-ray diffraction
2.72Å resolution

Crystal structure of a designed selected Ankyrin Repeat protein in complex with the phosphorylated MAP kinase ERK2

Released:
DOI: 10.2210/pdb3zuv/pdb
PDB entry 3zuv coloured by chain, front view.
PDB entry 3zuv coloured by chain, side view.
PDB entry 3zuv coloured by chain, top view.
Source organisms:
Primary publication:
Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries.
Kummer L, Parizek P, Rube P, Millgramm B, Prinz A, Mittl PR, Kaufholz M, Zimmermann B, Herberg FW, Plückthun A
Proc Natl Acad Sci U S A 109 E2248-57 (2012)
PMID: 22843676

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-158943 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mitogen-activated protein kinase 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 364 amino acids
Theoretical weight: 42.32 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P63086 (Residues: 3-358; Coverage: 99%)
Gene names: Erk2, Mapk, Mapk1, Prkm1
Sequence domains: Protein kinase domain
Structure domains:
DESIGNED ANKYRIN REPEAT PROTEIN Chains: B, D
Molecule details ›
Chains: B, D
Length: 136 amino acids
Theoretical weight: 14.75 KDa
Source organism: synthetic construct
Expression system: Escherichia coli K-12
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
2 modified residues:
Ligand TPO 2 x TPO
Ligand PTR 2 x PTR

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P212121
Unit cell:
a: 113.67Å b: 150.449Å c: 104.97Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.23
Expression systems:
  • Escherichia coli BL21(DE3)
  • Escherichia coli K-12

Quick links

Citations

18 review citations

Designed ankyrin repeat proteins (DARPins): binding proteins for research, diagnostics, and therapy.
Plückthun A. (2015)
17 more

6 mentions without citation

Structural and functional analysis of phosphorylation-specific binders of the kinase ERK from designed ankyrin repeat protein libraries.
Kummer et al. (2012)
5 more