› 3zul

X-ray diffraction
2.3Å resolution

Padron on (fluorescent) Icis intermediate state

Released:
DOI: 10.2210/pdb3zul/pdb
PDB 3zul coloured by chain and viewed from the front.
PDB 3zul coloured by chain and viewed from the side.
PDB 3zul coloured by chain and viewed from the top.
Source organism: Echinophyllia sp. SC22
Primary publication:
Low-temperature chromophore isomerization reveals the photoswitching mechanism of the fluorescent protein Padron.
Faro AR, Carpentier P, Jonasson G, Pompidor G, Arcizet D, Demachy I, Bourgeois D
J. Am. Chem. Soc. 133 16362-5 (2011)
PMID: 21923132

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fluorescent protein Dronpa Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 221 amino acids
Theoretical weight: 25.34 KDa
Source organism: Echinophyllia sp. SC22
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5TLG6 (Residues: 2-224; Coverage: 100%)
Gene name: Dronpa
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand GYC 6 x GYC

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: P21212
Unit cell:
a: 108.97Å b: 181.38Å c: 72.82Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.206 0.204 0.256
Expression system: Escherichia coli BL21

Quick links

Citations

10 review citations

Photoswitchable Fluorescent Proteins: Mechanisms on Ultrafast Timescales.
Tang et al. (2022)
9 more

1 mention without citation

The Positive Switching Fluorescent Protein Padron2 Enables Live-Cell Reversible Saturable Optical Linear Fluorescence Transitions (RESOLFT) Nanoscopy without Sequential Illumination Steps.
Konen et al. (2021)