› 3ztf

X-ray diffraction
1.31Å resolution

X-ray Structure of the Cyan Fluorescent Protein mTurquoise2 (K206A mutant)

Released:
DOI: 10.2210/pdb3ztf/pdb
PDB 3ztf coloured by chain and viewed from the front.
PDB 3ztf coloured by chain and viewed from the side.
PDB 3ztf coloured by chain and viewed from the top.
Source organism: Aequorea victoria
Primary publication:
Structure-guided evolution of cyan fluorescent proteins towards a quantum yield of 93%.
Goedhart J, von Stetten D, Noirclerc-Savoye M, Lelimousin M, Joosen L, Hink MA, van Weeren L, Gadella TW, Royant A
Nat Commun 3 751 (2012)
PMID: 22434194

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.7 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 2-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SWG 1 x SWG

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 51.01Å b: 62.549Å c: 70.356Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.134 0.132 0.177
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

39 review citations

Standardization of Synthetic Biology Tools and Assembly Methods for Saccharomyces cerevisiae and Emerging Yeast Species.
Malcı et al. (2022)
38 more

10 mentions without citation

Visual pH Sensors: From a Chemical Perspective to New Bioengineered Materials.
Di Costanzo et al. (2021)
9 more