3zpi

X-ray diffraction
1.63Å resolution

PikC D50N mutant in P21 space group

Released:
Source organism: Streptomyces venezuelae
Entry author: Podust LM

Function and Biology Details

Reaction catalysed:
10-deoxymethymycin + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+) + O(2) = methymycin + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytochrome P450 monooxygenase PikC Chains: A, B
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 48.27 KDa
Source organism: Streptomyces venezuelae
Expression system: Escherichia coli
UniProt:
  • Canonical: O87605 (Residues: 1-416; Coverage: 100%)
Gene names: picK, pikC
Sequence domains: Cytochrome P450
Structure domains: Cytochrome P450

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.3.1
Spacegroup: P21
Unit cell:
a: 76.085Å b: 59.784Å c: 91.267Å
α: 90° β: 95.81° γ: 90°
R-values:
R R work R free
0.176 0.174 0.219
Expression system: Escherichia coli