3zo7

X-ray diffraction
2.22Å resolution

Crystal structure of ClcFE27A with substrate

Released:
Source organism: Rhodococcus opacus
Entry authors: Roth C, Groening JAD, Kaschabek SR, Schloemann M, Straeter N

Function and Biology Details

Reaction catalysed:
(+)-muconolactone = 5-oxo-4,5-dihydrofuran-2-acetate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo decamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Muconolactone Delta-isomerase Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 94 amino acids
Theoretical weight: 11.15 KDa
Source organism: Rhodococcus opacus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8G9L0 (Residues: 1-94; Coverage: 100%)
Gene names: R1CP_39625, clcF
Sequence domains: Muconolactone delta-isomerase
Structure domains: Dimeric alpha+beta barrel

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 77.836Å b: 103.112Å c: 137.767Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.186 0.232
Expression system: Escherichia coli