X-ray diffraction
2.07Å resolution

Crystal structure of Mycoplasma mobile Leucyl-tRNA Synthetase with Leu-AMS in the active site

Source organism: Mesomycoplasma mobile
Entry authors: Li L, Palencia A, Lukk T, Li Z, Luthey-Schulten ZA, Cusack S, Martinis SA, Boniecki MT

Function and Biology Details

Reaction catalysed:
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Leucine--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 637 amino acids
Theoretical weight: 75.13 KDa
Source organism: Mesomycoplasma mobile
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q6KHA5 (Residues: 1-630; Coverage: 100%)
Gene names: MMOB5390, leuS
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: R32
Unit cell:
a: 202.851Å b: 202.851Å c: 213.922Å
α: 90° β: 90° γ: 120°
R R work R free
0.162 0.16 0.199
Expression system: Escherichia coli BL21(DE3)