3zfq

X-ray diffraction
2.65Å resolution

Crystal structure of product-like, processed N-terminal protease Npro with mercury

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-177434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase C53 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 148 amino acids
Theoretical weight: 16.56 KDa
Source organism: Pestivirus strain D32/00_HoBi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5L4B1 (Residues: 22-168; Coverage: 88%)
Gene name: Npro
Sequence domains: Pestivirus Npro endopeptidase C53
Structure domains: Pestivirus Npro endopeptidase C53, interaction domain

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21
Unit cell:
a: 41.64Å b: 41.23Å c: 43.21Å
α: 90° β: 114.15° γ: 90°
R-values:
R R work R free
0.211 0.208 0.276
Expression system: Escherichia coli BL21(DE3)