3zfo

X-ray diffraction
2.01Å resolution

Crystal structure of substrate-like, unprocessed N-terminal protease Npro mutant S169P

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
PDBe Complex ID:
PDB-CPX-177434 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase C53 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 164 amino acids
Theoretical weight: 18.4 KDa
Source organism: Pestivirus strain D32/00_HoBi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5L4B1 (Residues: 22-168; Coverage: 88%)
Gene name: Npro
Sequence domains: Pestivirus Npro endopeptidase C53
Structure domains: Pestivirus Npro endopeptidase C53, interaction domain

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: P21
Unit cell:
a: 43.33Å b: 41.05Å c: 46.25Å
α: 90° β: 98.95° γ: 90°
R-values:
R R work R free
0.203 0.2 0.255
Expression system: Escherichia coli BL21(DE3)