3zet

X-ray diffraction
2.31Å resolution

Function and Biology Details

Reaction catalysed:
L-threonylcarbamoyladenylate + adenine(37) in tRNA = AMP + N(6)-L-threonylcarbamoyladenine(37) in tRNA
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
t(6)A37 threonylcarbamoyladenosine biosynthesis protein TsaB Chain: A
Molecule details ›
Chain: A
Length: 229 amino acids
Theoretical weight: 24.51 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. ST4/74
Expression system: Escherichia coli
UniProt:
  • Canonical: E8X8J1 (Residues: 1-229; Coverage: 99%)
Gene names: STM474_1842, yeaz
Sequence domains: tRNA N6-adenosine threonylcarbamoyltransferase
Structure domains: Nucleotidyltransferase; domain 5
tRNA N6-adenosine threonylcarbamoyltransferase Chain: B
Molecule details ›
Chain: B
Length: 337 amino acids
Theoretical weight: 35.98 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. ST4/74
Expression system: Escherichia coli
UniProt:
  • Canonical: E8XBD7 (Residues: 1-337; Coverage: 100%)
Gene names: STM474_3362, gcp, tsaD
Sequence domains: tRNA N6-adenosine threonylcarbamoyltransferase
Structure domains: Nucleotidyltransferase; domain 5

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: C2
Unit cell:
a: 121.152Å b: 61.663Å c: 83.835Å
α: 90° β: 101.14° γ: 90°
R-values:
R R work R free
0.181 0.178 0.235
Expression system: Escherichia coli