3zdn

X-ray diffraction
2.55Å resolution

D11-C mutant of monoamine oxidase from Aspergillus niger

Released:
Source organism: Aspergillus niger
Entry authors: Frank A, Ghislieri D, Willies S, Turner NJ, Grogan G

Function and Biology Details

Reaction catalysed:
RCH(2)NHR' + H(2)O + O(2) = RCHO + R'NH(2) + H(2)O(2)
Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monoamine oxidase N Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 495 amino acids
Theoretical weight: 55.37 KDa
Source organism: Aspergillus niger
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46882 (Residues: 1-495; Coverage: 100%)
Gene name: maoN
Sequence domains: Flavin containing amine oxidoreductase
Structure domains:

Ligands and Environments


Cofactor: Ligand FAD 4 x FAD
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 108.6Å b: 119.34Å c: 178.78Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.153 0.214
Expression system: Escherichia coli BL21(DE3)