› 3zcn

X-ray diffraction
1.7Å resolution

Fic protein from SHEWANELLA ONEIDENSIS in complex with ATP

Released:
DOI: 10.2210/pdb3zcn/pdb
PDB 3zcn coloured by chain and viewed from the front.
PDB 3zcn coloured by chain and viewed from the side.
PDB 3zcn coloured by chain and viewed from the top.
Source organism: Shewanella oneidensis
Primary publication:
Conserved inhibitory mechanism and competent ATP binding mode for adenylyltransferases with fic fold.
Goepfert A, Stanger FV, Dehio C, Schirmer T
PLoS ONE 8 e64901 (2013)
PMID: 23738009

Function and Biology Details

Reaction catalysed: 
ATP + [protein]-L-threonine = diphosphate + [protein]-O(4)-(5'-adenylyl)-L-threonine
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein adenylyltransferase SoFic Chains: A, B
Molecule details ›
Chains: A, B
Length: 378 amino acids
Theoretical weight: 43.14 KDa
Source organism: Shewanella oneidensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8E9K5 (Residues: 2-372; Coverage: 100%)
Gene names: SO_4266, fic
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ATP 2 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P21
Unit cell:
a: 37.79Å b: 164.91Å c: 70.23Å
α: 90° β: 94.43° γ: 90°
R-values:
R R work R free
0.168 0.166 0.202
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

5 review citations

Enzymes Involved in AMPylation and deAMPylation.
Casey et al. (2018)
4 more

1 mention without citation

Conserved inhibitory mechanism and competent ATP binding mode for adenylyltransferases with Fic fold.
Goepfert et al. (2013)