X-ray diffraction
1.49Å resolution

Ascorbate peroxidase W41A-H42C mutant

Source organism: Glycine max
Entry authors: Gumiero A, Raven EL, Moody PCE

Function and Biology Details

Reaction catalysed:
(1a) 2 L-ascorbate + H(2)O(2) + 2 H(+) = 2 monodehydroascorbate + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
L-ascorbate peroxidase Chain: A
Molecule details ›
Chain: A
Length: 261 amino acids
Theoretical weight: 28.21 KDa
Source organism: Glycine max
Expression system: Escherichia coli
  • Canonical: Q43758 (Residues: 2-250; Coverage: 100%)
Gene name: apx1
Sequence domains: Peroxidase
Structure domains:

Ligands and Environments

Cofactor: Ligand HEM 1 x HEM
3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P42212
Unit cell:
a: 82.27Å b: 82.27Å c: 75.31Å
α: 90° β: 90° γ: 90°
R R work R free
0.14 0.138 0.172
Expression system: Escherichia coli