› 3wug

X-ray diffraction
1.88Å resolution

The mutant crystal structure of b-1,4-Xylanase (XynAS9_V43P/G44E) with xylobiose from Streptomyces sp. 9

Released:
DOI: 10.2210/pdb3wug/pdb
PDB entry 3wug coloured by chain, front view.
PDB entry 3wug coloured by chain, side view.
PDB entry 3wug coloured by chain, top view.
Source organism: Streptomyces sp.
Primary publication:
Structural perspectives of an engineered β-1,4-xylanase with enhanced thermostability.
Chen CC, Luo H, Han X, Lv P, Ko TP, Peng W, Huang CH, Wang K, Gao J, Zheng Y, Yang Y, Zhang J, Yao B, Guo RT
J Biotechnol 189 175-82 (2014)
PMID: 25193708

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110153 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Endo-1,4-beta-xylanase A Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 34.68 KDa
Source organism: Streptomyces sp.
Expression system: Komagataella pastoris
UniProt:
  • Canonical: B4XVN1 (Residues: 39-351; Coverage: 73%)
Gene name: xynAS9
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

Carbohydrate polymer : NEW Components: XYP
Carbohydrate polymer
1 bound ligand:
Ligand ZN 7 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P6522
Unit cell:
a: 80.798Å b: 80.798Å c: 288.589Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.2 0.2 0.215
Expression system: Komagataella pastoris

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Citations

2 review citations

Engineering Thermostable Microbial Xylanases Toward its Industrial Applications.
Kumar et al. (2018)
1 more

1 mention without citation

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Moya-Mendez et al. (2021)