› 3wl4

X-ray diffraction
1.54Å resolution

N,N'-diacetylchitobiose deacetylase (Se-derivative) from Pyrococcus furiosus

Released:
DOI: 10.2210/pdb3wl4/pdb
PDB entry 3wl4 coloured by chain, front view.
PDB entry 3wl4 coloured by chain, side view.
PDB entry 3wl4 coloured by chain, top view.
Source organism: Pyrococcus furiosus DSM 3638
Primary publication:
Expression from engineered Escherichia coli chromosome and crystallographic study of archaeal N,N'-diacetylchitobiose deacetylase.
Mine S, Niiyama M, Hashimoto W, Ikegami T, Koma D, Ohmoto T, Fukuda Y, Inoue T, Abe Y, Ueda T, Morita J, Uegaki K, Nakamura T
FEBS J 281 2584-96 (2014)
PMID: 24702737

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186481 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
PIG-L family deacetylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 267 amino acids
Theoretical weight: 31.39 KDa
Source organism: Pyrococcus furiosus DSM 3638
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8U3V1 (Residues: 2-267; Coverage: 100%)
Gene name: PF0354
Sequence domains: GlcNAc-PI de-N-acetylase
Structure domains: LmbE-like

Ligands and Environments

6 bound ligands:
Ligand CD 4 x CD
Ligand CA 2 x CA
Ligand CL 8 x CL
Ligand TAM 4 x TAM
Ligand HEZ 21 x HEZ
Ligand HE2 2 x HE2
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: R3
Unit cell:
a: 113.894Å b: 113.894Å c: 201.029Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.154 0.154 0.169
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Chitin Deacetylases: Structures, Specificities, and Biotech Applications.
Grifoll-Romero et al. (2018)
2 more

3 mentions without citation

Expression from engineered Escherichia coli chromosome and crystallographic study of archaeal N,N'-diacetylchitobiose deacetylase.
Mine et al. (2014)
2 more