› 3wis

X-ray diffraction
1.9Å resolution

Crystal structure of Burkholderia xenovorans DmrB in complex with FMN: A Cubic Protein Cage for Redox Transfer

Released:
DOI: 10.2210/pdb3wis/pdb
PDB entry 3wis coloured by chain, front view.
PDB entry 3wis coloured by chain, side view.
PDB entry 3wis coloured by chain, top view.
Source organism: Paraburkholderia xenovorans LB400
Primary publication:
Structure of dihydromethanopterin reductase, a cubic protein cage for redox transfer.
McNamara DE, Cascio D, Jorda J, Bustos C, Wang TC, Rasche ME, Yeates TO, Bobik TA
J Biol Chem 289 8852-64 (2014)
PMID: 24523405

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo 24-mer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171877 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Flavoprotein domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 199 amino acids
Theoretical weight: 22.1 KDa
Source organism: Paraburkholderia xenovorans LB400
Expression system: Escherichia coli
UniProt:
  • Canonical: Q13QT8 (Residues: 1-192; Coverage: 100%)
Gene name: Bxe_B2440
Sequence domains: Flavoprotein
Structure domains: Flavin prenyltransferase-like

Ligands and Environments

2 bound ligands:
Ligand FMN 2 x FMN
Ligand SO4 4 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: F432
Unit cell:
a: 183.84Å b: 183.84Å c: 183.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.188
Expression system: Escherichia coli

Quick links

Citations

1 review citation

SEDPHAT--a platform for global ITC analysis and global multi-method analysis of molecular interactions.
Zhao et al. (2015)
2 other articles

3 mentions without citation

Crystal structure of UbiX, an aromatic acid decarboxylase from the psychrophilic bacterium Colwellia psychrerythraea that undergoes FMN-induced conformational changes.
Do et al. (2015)
2 more