› 3wfl

X-ray diffraction
1.6Å resolution

Crtstal structure of glycoside hydrolase family 5 beta-mannanase from Talaromyces trachyspermus

Released:
DOI: 10.2210/pdb3wfl/pdb
PDB entry 3wfl coloured by chain, front view.
PDB entry 3wfl coloured by chain, side view.
PDB entry 3wfl coloured by chain, top view.
Source organism: Talaromyces trachyspermus
Entry authors: Suzuki K, Ichinose H, Kamino K, Ogasawara W, Kaneko S, Fushinobu S

Function and Biology Details

Reaction catalysed: 
Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-100597 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
mannan endo-1,4-beta-mannosidase Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 37.1 KDa
Source organism: Talaromyces trachyspermus
UniProt:
  • Canonical: A0A087WNH1 (Residues: 1-342; Coverage: 100%)
Sequence domains: Cellulase (glycosyl hydrolase family 5)
Structure domains: Glycosidases

Ligands and Environments

4 bound ligands:
Ligand NAG 1 x NAG
Ligand TRS 1 x TRS
Ligand GOL 7 x GOL
Ligand PO4 2 x PO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-5A
Spacegroup: P212121
Unit cell:
a: 58.434Å b: 75.972Å c: 79.723Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.115 0.114 0.138

Quick links