3wdu

X-ray diffraction
2.25Å resolution

The complex structure of PtLic16A with cellobiose

Released:
Entry authors: Cheng YS, Huang CH, Chen CC, Huang TY, Ko TP, Huang JW, Wu TH, Liu JR, Guo RT

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
GH16 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 297 amino acids
Theoretical weight: 32.1 KDa
Source organism: Paecilomyces sp. 'thermophila'
Expression system: Komagataella pastoris
UniProt:
  • Canonical: E0XN39 (Residues: 19-314; Coverage: 100%)
Sequence domains: Glycosyl hydrolases family 16
Structure domains: Jelly Rolls

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: C2221
Unit cell:
a: 88.142Å b: 172.559Å c: 166.376Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.181 0.213
Expression system: Komagataella pastoris