3wby

X-ray diffraction
3.2Å resolution

Crystal structure of Gox0644 D53A mutant in complex with NADPH

Released:
Source organism: Gluconobacter oxydans 621H
Entry authors: Yuan YA, Wang C

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldo_ket_red domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 282 amino acids
Theoretical weight: 32.01 KDa
Source organism: Gluconobacter oxydans 621H
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5FT75 (Residues: 1-279; Coverage: 100%)
Gene name: GOX0644
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 55.638Å b: 76.587Å c: 125.624Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.224 0.218 0.326
Expression system: Escherichia coli