3wbk

X-ray diffraction
3.3Å resolution

crystal structure analysis of eukaryotic translation initiation factor 5B and 1A complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
monomeric
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Eukaryotic translation initiation factor 5B Chains: A, B
Molecule details ›
Chains: A, B
Length: 606 amino acids
Theoretical weight: 67.72 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P39730 (Residues: 401-1002; Coverage: 60%)
Gene names: FUN12, YAL035W
Sequence domains:
Structure domains:
Eukaryotic translation initiation factor 1A Chain: C
Molecule details ›
Chain: C
Length: 131 amino acids
Theoretical weight: 15.04 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38912 (Residues: 27-153; Coverage: 83%)
Gene names: TIF11, YM8156.02C, YMR260C
Sequence domains: Translation initiation factor 1A / IF-1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P212121
Unit cell:
a: 101.939Å b: 120.942Å c: 132.75Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.261 0.257 0.317
Expression system: Escherichia coli