3w5o

X-ray diffraction
2.84Å resolution

Crystal Structure of Human DNA ligase IV

Released:
Source organism: Homo sapiens
Entry authors: Gu X, Ochi T, Blundell TL

Function and Biology Details

Reaction catalysed:
(1a) ATP + [DNA ligase]-L-lysine = [DNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA ligase 4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 610 amino acids
Theoretical weight: 69.54 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P49917 (Residues: 1-609; Coverage: 67%)
Gene name: LIG4
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P21
Unit cell:
a: 68.29Å b: 104.36Å c: 120.04Å
α: 90° β: 94.08° γ: 90°
R-values:
R R work R free
0.196 0.194 0.246
Expression system: Escherichia coli