› 3w30

X-ray diffraction
2.99Å resolution

Structual basis for the recognition of Ubc13 by the Shigella flexneri effector OspI

Released:
DOI: 10.2210/pdb3w30/pdb
PDB entry 3w30 coloured by chain, front view.
PDB entry 3w30 coloured by chain, side view.
PDB entry 3w30 coloured by chain, top view.
Source organism: Shigella flexneri
Primary publication:
Structural basis for the recognition of Ubc13 by the Shigella flexneri effector OspI.
Nishide A, Kim M, Takagi K, Himeno A, Sanada T, Sasakawa C, Mizushima T
J Mol Biol 425 2623-31 (2013)
PMID: 23542009

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-186623 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ORF169b Chains: A, B
Molecule details ›
Chains: A, B
Length: 220 amino acids
Theoretical weight: 24.12 KDa
Source organism: Shigella flexneri
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8VSD5 (Residues: 1-212; Coverage: 100%)
Gene names: ORF169b, SF_p0209
Sequence domains: Shigella glutamine deamidase OspI
Structure domains: Cathepsin B; Chain A

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P212121
Unit cell:
a: 67.583Å b: 67.352Å c: 84.84Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.23 0.303
Expression system: Escherichia coli

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Citations

11 review citations

How Do the Virulence Factors of Shigella Work Together to Cause Disease?
Mattock et al. (2017)
10 more