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Crystal structure of Saccharomyces cerevisiae Atg8 complexed with Atg32 AIM

X-ray diffraction
3Å resolution
PDB 3vxw coloured by chain and viewed from the front.
PDB 3vxw coloured by chain and viewed from the side.
PDB 3vxw coloured by chain and viewed from the top.
Source organism: Saccharomyces cerevisiae S288C
Assembly composition:
hetero dimer (preferred)
Entry contents: 2 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero dimer
Accessible surface area: 6700 Å2
Buried surface area: 1300 Å2
Dissociation area: 450 Å2
Dissociation energy (ΔGdiss): 4 kcal/mol
Dissociation entropy (TΔSdiss): 6 kcal/mol
Interface energy (ΔGint): -36 kcal/mol
Symmetry number: 1

Macromolecules

Autophagy-related protein 8
Chain: A
Length: 119 amino acids
Theoretical weight: 13.76 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38182 (Residues: 1-116; Coverage: 99%)
Gene names: APG8, ATG8, AUT7, CVT5, YBL0732, YBL078C
Pfam: Autophagy protein Atg8 ubiquitin like
InterPro:
CATH: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
PDB 3vxw contains 1 copy of Autophagy-related protein 8 in assembly 1. This protein is highlighted and viewed from the front.
PDB 3vxw contains 1 copy of Autophagy-related protein 8 in assembly 1. This protein is highlighted and viewed from the side.
PDB 3vxw contains 1 copy of Autophagy-related protein 8 in assembly 1. This protein is highlighted and viewed from the top.
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Autophagy-related protein 32
Chain: B
Length: 6 amino acids
Theoretical weight: 732 Da
Source organism: Saccharomyces cerevisiae S288C
Expression system: Not provided
UniProt:
  • Canonical: P40458 (Residues: 85-90; Coverage: 1%)
Gene names: ATG32, ECM17, YIL146C
PDB 3vxw contains 1 copy of Autophagy-related protein 32 in assembly 1. This protein is highlighted and viewed from the front.
PDB 3vxw contains 1 copy of Autophagy-related protein 32 in assembly 1. This protein is highlighted and viewed from the side.
PDB 3vxw contains 1 copy of Autophagy-related protein 32 in assembly 1. This protein is highlighted and viewed from the top.
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