3vnm

X-ray diffraction
2.12Å resolution

Crystal structures of D-Psicose 3-epimerase with D-sorbose from Clostridium cellulolyticum H10

Released:

Function and Biology Details

Reaction catalysed:
D-psicose = D-fructose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-psicose 3-epimerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 293 amino acids
Theoretical weight: 33.08 KDa
Source organism: Ruminiclostridium cellulolyticum H10
Expression system: Escherichia coli
UniProt:
  • Canonical: B8I944 (Residues: 1-293; Coverage: 100%)
Gene name: Ccel_0941
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P21
Unit cell:
a: 79.924Å b: 115.225Å c: 91.614Å
α: 90° β: 105.73° γ: 90°
R-values:
R R work R free
0.19 0.19 0.232
Expression system: Escherichia coli