3vnj

X-ray diffraction
2.08Å resolution

Crystal structures of D-Psicose 3-epimerase with D-psicose from Clostridium cellulolyticum H10

Released:
Entry authors: Chan HC, Zhu Y, Hu Y, Ko TP, Huang CH, Ren F, Chen CC, Guo RT, Sun Y

Function and Biology Details

Reaction catalysed:
D-psicose = D-fructose
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-psicose 3-epimerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 294 amino acids
Theoretical weight: 33.15 KDa
Source organism: Ruminiclostridium cellulolyticum H10
Expression system: Escherichia coli
UniProt:
  • Canonical: B8I944 (Residues: 1-293; Coverage: 100%)
Gene name: Ccel_0941
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13C1
Spacegroup: P21
Unit cell:
a: 79.883Å b: 115.499Å c: 91.577Å
α: 90° β: 105.16° γ: 90°
R-values:
R R work R free
0.182 0.182 0.22
Expression system: Escherichia coli