› 3vn5

X-ray diffraction
1.98Å resolution

Crystal structure of Aquifex aeolicus RNase H3

Released:
DOI: 10.2210/pdb3vn5/pdb
PDB entry 3vn5 coloured by chain, front view.
PDB entry 3vn5 coloured by chain, side view.
PDB entry 3vn5 coloured by chain, top view.
Source organism: Aquifex aeolicus VF5
Primary publication:
Structure and characterization of RNase H3 from Aquifex aeolicus.
Jongruja N, You DJ, Angkawidjaja C, Kanaya E, Koga Y, Kanaya S
FEBS J 279 2737-53 (2012)
PMID: 22686566

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130525 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease HIII Chain: A
Molecule details ›
Chain: A
Length: 257 amino acids
Theoretical weight: 29.58 KDa
Source organism: Aquifex aeolicus VF5
Expression system: Escherichia coli
UniProt:
  • Canonical: O67644 (Residues: 1-257; Coverage: 100%)
Gene names: aq_1768, rnhC
Sequence domains: Ribonuclease HII
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL44XU
Spacegroup: P3121
Unit cell:
a: 48.943Å b: 48.943Å c: 293.217Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.206 0.204 0.238
Expression system: Escherichia coli

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Citations

1 review citation

Ribonucleotides in bacterial DNA.
Schroeder et al. (2015)
5 other articles

1 mention without citation

Alternative dimerization is required for activity and inhibition of the HEPN ribonuclease RnlA.
Garcia-Rodriguez et al. (2021)