3vmk

X-ray diffraction
1.48Å resolution

3-isopropylmalate dehydrogenase from Shewanella benthica DB21 MT-2

Released:

Function and Biology Details

Reaction catalysed:
(1a) (2R,3S)-3-isopropylmalate + NAD(+) = (2S)-2-isopropyl-3-oxosuccinate + NADH
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-isopropylmalate dehydrogenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 40.68 KDa
Source organism: Shewanella benthica
Expression system: Escherichia coli
UniProt:
  • Canonical: D2YZL2 (Residues: 2-364; Coverage: 100%)
Gene names: SHEWBE_0188, leuB
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NE3A
Spacegroup: P21
Unit cell:
a: 51.972Å b: 59.208Å c: 119.779Å
α: 90° β: 95.12° γ: 90°
R-values:
R R work R free
0.195 0.193 0.232
Expression system: Escherichia coli