3vl6

X-ray diffraction
2.07Å resolution

3-isopropylmalate dehydrogenase from Shewanella oneidensis MR-1 at 580 MPa

Released:
Source organism: Shewanella oneidensis MR-1
Primary publication:
High-pressure-induced water penetration into 3-isopropylmalate dehydrogenase.
Acta Crystallogr. D Biol. Crystallogr. 68 300-9 (2012)
PMID: 22349232

Function and Biology Details

Reaction catalysed:
(1a) (2R,3S)-3-isopropylmalate + NAD(+) = (2S)-2-isopropyl-3-oxosuccinate + NADH
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-isopropylmalate dehydrogenase Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 40.69 KDa
Source organism: Shewanella oneidensis MR-1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8E9N3 (Residues: 2-364; Coverage: 100%)
Gene names: SO_4235, leuB
Sequence domains: Isocitrate/isopropylmalate dehydrogenase
Structure domains: Isopropylmalate Dehydrogenase

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: C2
Unit cell:
a: 103.144Å b: 57.38Å c: 75.213Å
α: 90° β: 118.76° γ: 90°
R-values:
R R work R free
0.177 0.174 0.235
Expression system: Escherichia coli