PDBe 3vhx

X-ray diffraction
2.81Å resolution

The crystal structure of Arf6-MKLP1 (Mitotic kinesin-like protein 1) complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ADP-ribosylation factor 6 Chains: A, C, E, G
Molecule details ›
Chains: A, C, E, G
Length: 172 amino acids
Theoretical weight: 19.99 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P62331 (Residues: 13-175; Coverage: 93%)
Gene name: Arf6
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Kinesin-like protein KIF23 Chains: B, D, F, H
Molecule details ›
Chains: B, D, F, H
Length: 120 amino acids
Theoretical weight: 13.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q02241 (Residues: 794-911; Coverage: 12%)
Gene names: KIF23, KNSL5, MKLP1
Sequence domains: Arf6-interacting domain of mitotic kinesin-like protein 1
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE AR-NW12A
Spacegroup: P21
Unit cell:
a: 45.743Å b: 174.573Å c: 76.814Å
α: 90° β: 98.74° γ: 90°
R-values:
R R work R free
0.262 0.201 0.232
Expression system: Escherichia coli