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Crystal structure of GFP-Wrnip1 UBZ domain fusion protein in complex with ubiquitin

X-ray diffraction
2.4Å resolution
PDB entry 3vht coloured by chain, front view.
PDB entry 3vht coloured by chain, side view.
PDB entry 3vht coloured by chain, top view.
Source organisms:
Assembly composition:
hetero trimer (preferred)
Entry contents: 3 distinct polypeptide molecules

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Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero trimer
Accessible surface area: 24275.73 Å2
Buried surface area: 3270.61 Å2
Dissociation area: 687.53 Å2
Dissociation energy (ΔGdiss): -2.02 kcal/mol
Dissociation entropy (TΔSdiss): 10.84 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-154528

Macromolecules

Green fluorescent protein
Chain: A
Length: 233 amino acids
Theoretical weight: 26.31 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-230; Coverage: 97%)
Gene name: GFP
Pfam: Green fluorescent protein
InterPro:
CATH: Green fluorescent protein
Green fluorescent protein in PDB entry 3vht, assembly 1, front view.
Green fluorescent protein in PDB entry 3vht, assembly 1, side view.
Green fluorescent protein in PDB entry 3vht, assembly 1, top view.
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ATPase WRNIP1; Green fluorescent protein
Chain: B
Length: 271 amino acids
Theoretical weight: 30.54 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-230; Coverage: 97%)
  • Canonical: Q96S55 (Residues: 9-46; Coverage: 6%)
    • nullnull
Gene names: GFP, WHIP, WRNIP1
Pfam:
InterPro:
CATH:
ATPase WRNIP1 in PDB entry 3vht, assembly 1, front view.
ATPase WRNIP1 in PDB entry 3vht, assembly 1, side view.
ATPase WRNIP1 in PDB entry 3vht, assembly 1, top view.
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Ubiquitin
Chain: C
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Mus musculus
UniProt:
  • Canonical: P62983 (Residues: 1-76; Coverage: 49%)
Gene names: Rps27a, Uba80, Ubcep1
Pfam: Ubiquitin family
InterPro:
CATH: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1
Ubiquitin in PDB entry 3vht, assembly 1, front view.
Ubiquitin in PDB entry 3vht, assembly 1, side view.
Ubiquitin in PDB entry 3vht, assembly 1, top view.
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