PDBe 3val

X-ray diffraction
2.5Å resolution

Structure of U2AF65 variant with BrU5C1 DNA


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Splicing factor U2AF 65 kDa subunit Chains: A, B, D, I
Molecule details ›
Chains: A, B, D, I
Length: 174 amino acids
Theoretical weight: 19.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P26368 (Residues: 148-336; Coverage: 36%)
Gene names: U2AF2, U2AF65
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits
DNA (5'-D(*C*UP*UP*UP*(BRU)P*UP*U)-3') Chains: E, H, K, P
Molecule details ›
Chains: E, H, K, P
Length: 7 nucleotides
Theoretical weight: 2.06 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P21
Unit cell:
a: 37.583Å b: 137.081Å c: 83.962Å
α: 90° β: 102.97° γ: 90°
R R work R free
0.208 0.208 0.271
Expression systems:
  • Escherichia coli
  • Not provided