PDBe 3va4

X-ray diffraction
1.54Å resolution

Crystal structure of the mammalian MDC1 FHA domain complexed with CHK2 pThr68 peptide

Source organisms:
Primary publication:
Structural delineation of MDC1-FHA domain binding with CHK2-pThr68.
Biochemistry 51 575-7 (2012)
PMID: 22211259

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Mediator of DNA damage checkpoint protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 132 amino acids
Theoretical weight: 14.59 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
  • Canonical: Q5PSV9 (Residues: 29-139; Coverage: 7%)
Gene names: Kiaa0170, Mdc1
Sequence domains: FHA domain
Structure domains: Tumour Suppressor Smad4
Serine/threonine-protein kinase Chk2 Chain: C
Molecule details ›
Chain: C
Length: 11 amino acids
Theoretical weight: 1.35 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: O96017 (Residues: 63-73; Coverage: 2%)
Gene names: CDS1, CHEK2, CHK2, RAD53

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSRRC BEAMLINE BL13B1
Spacegroup: P212121
Unit cell:
a: 28.508Å b: 70.224Å c: 101.335Å
α: 90° β: 90° γ: 90°
R R work R free
0.162 0.159 0.205
Expression systems:
  • Escherichia coli
  • Not provided