3v9k

X-ray diffraction
1.5Å resolution

Crystal structure of mouse 1-pyrroline-5-carboxylate dehydrogenase complexed with the product glutamate

Released:
Source organism: Mus musculus
Entry authors: Tanner JJ, Srivastava D

Function and Biology Details

Reaction catalysed:
L-glutamate 5-semialdehyde + NAD(+) + H(2)O = L-glutamate + NADH
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Delta-1-pyrroline-5-carboxylate dehydrogenase, mitochondrial Chains: A, B
Molecule details ›
Chains: A, B
Length: 563 amino acids
Theoretical weight: 61.95 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8CHT0 (Residues: 21-562; Coverage: 96%)
Gene name: Aldh4a1
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P212121
Unit cell:
a: 84.82Å b: 93.954Å c: 132.241Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.152 0.151 0.169
Expression system: Escherichia coli BL21(DE3)