3v99

X-ray diffraction
2.25Å resolution

S663D Stable-5-LOX in complex with Arachidonic Acid

Released:
Source organism: Homo sapiens
Entry authors: Gilbert NC, Rui Z, Neau DB, Waight M, Bartlett SG, Boeglin WE, Brash AR, Newcomer ME

Function and Biology Details

Reaction catalysed:
(1a) arachidonate + O(2) = (5S,6E,8Z,11Z,14Z)-5-hydroperoxyicosa-6,8,11,14-tetraenoate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Polyunsaturated fatty acid 5-lipoxygenase Chains: A, B
Molecule details ›
Chains: A, B
Length: 691 amino acids
Theoretical weight: 79.46 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09917 (Residues: 1-674; Coverage: 100%)
Gene names: ALOX5, LOG5
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P21
Unit cell:
a: 51.755Å b: 200.844Å c: 72.184Å
α: 90° β: 105.65° γ: 90°
R-values:
R R work R free
0.188 0.186 0.233
Expression system: Escherichia coli