Function and Biology Details
Reaction catalysed:
Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
homo tetradecamer (preferred)
Assembly name:
ATP-dependent Clp protease proteolytic subunit (preferred)
PDBe Complex ID:
PDB-CPX-173494 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
SLS BEAMLINE X06SA
Spacegroup:
P21
Expression system: Escherichia coli BL21(DE3)