3v4p

X-ray diffraction
3.15Å resolution

crystal structure of a4b7 headpiece complexed with Fab ACT-1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (5 distinct):
Integrin alpha-4 Chains: A, C
Molecule details ›
Chains: A, C
Length: 597 amino acids
Theoretical weight: 65.64 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P13612 (Residues: 34-620; Coverage: 59%)
Gene names: CD49D, ITGA4
Sequence domains: FG-GAP repeat
Structure domains:
Integrin beta-7 Chains: B, D
Molecule details ›
Chains: B, D
Length: 503 amino acids
Theoretical weight: 55.43 KDa
Source organism: Homo sapiens
Expression system: Cricetulus griseus
UniProt:
  • Canonical: P26010 (Residues: 20-512; Coverage: 63%)
Gene name: ITGB7
Sequence domains:
Structure domains:
MONOCLONAL ANTIBODY Act-1 HEAVY CHAIN Chains: H, M
Molecule details ›
Chains: H, M
Length: 219 amino acids
Theoretical weight: 23.57 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins
MONOCLONAL ANTIBODY Act-1 LIGHT CHAIN Chains: L, N
Molecule details ›
Chains: L, N
Length: 217 amino acids
Theoretical weight: 23.9 KDa
Source organism: Mus musculus
Expression system: Mus musculus
Structure domains: Immunoglobulins

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P21
Unit cell:
a: 137.78Å b: 122.74Å c: 158.14Å
α: 90° β: 115.38° γ: 90°
R-values:
R R work R free
0.22 0.22 0.251
Expression systems:
  • Cricetulus griseus
  • Mus musculus