3v0x

X-ray diffraction
1.9Å resolution

Bovine trypsin variant X(tripleGlu217Phe227) in complex with small molecule inhibitor

Released:
Source organism: Bos taurus
Entry authors: Tziridis A, Neumann P, Kolenko P, Stubbs MT

Function and Biology Details

Reaction catalysed:
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease 1 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.48 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 54.494Å b: 57.478Å c: 67.003Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.159 0.207
Expression system: Escherichia coli BL21(DE3)