› 3v0x

X-ray diffraction
1.9Å resolution

Bovine trypsin variant X(tripleGlu217Phe227) in complex with small molecule inhibitor

Released:
DOI: 10.2210/pdb3v0x/pdb
PDB 3v0x coloured by chain and viewed from the front.
PDB 3v0x coloured by chain and viewed from the side.
PDB 3v0x coloured by chain and viewed from the top.
Source organism: Bos taurus
Entry authors: Tziridis A, Neumann P, Kolenko P, Stubbs MT

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease 1 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.48 KDa
Source organism: Bos taurus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:
Ligand CA 1 x CA
Ligand ANH 1 x ANH
Ligand GOL 1 x GOL
1 modified residue:
Ligand DSG 1 x DSG

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P212121
Unit cell:
a: 54.494Å b: 57.478Å c: 67.003Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.165 0.159 0.207
Expression system: Escherichia coli BL21(DE3)

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