› 3uz5

X-ray diffraction
1.9Å resolution

Designed protein KE59 R13 3/11H

Released:
DOI: 10.2210/pdb3uz5/pdb
PDB 3uz5 coloured by chain and viewed from the front.
PDB 3uz5 coloured by chain and viewed from the side.
PDB 3uz5 coloured by chain and viewed from the top.
Source organism: Escherichia coli
Entry authors: Khersonsky O, Kiss G, Roethlisberger D, Dym O, Albeck S, Houk KN, Baker D, Tawfik DS, Israel Structural Proteomics Center (ISPC)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Kemp eliminase KE59 R13 3/11H Chains: A, B
Molecule details ›
Chains: A, B
Length: 252 amino acids
Theoretical weight: 28.76 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
Structure domains: Aldolase class I

Ligands and Environments

3 bound ligands:
Ligand 0CU 2 x 0CU
Ligand PO4 4 x PO4
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 95.476Å b: 123.744Å c: 45.702Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.184 0.251
Expression system: Escherichia coli

Quick links

Citations

40 review citations

Catalytic Principles from Natural Enzymes and Translational Design Strategies for Synthetic Catalysts.
Li et al. (2021)
39 more

1 mention without citation

Bridging the gaps in design methodologies by evolutionary optimization of the stability and proficiency of designed Kemp eliminase KE59.
Khersonsky et al. (2012)