3uxy

X-ray diffraction
2.1Å resolution

The crystal structure of short chain dehydrogenase from Rhodobacter sphaeroides

Released:
Entry authors: Zhang Z, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, LaFleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Short-chain dehydrogenase/reductase SDR Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 266 amino acids
Theoretical weight: 27.51 KDa
Source organism: Cereibacter sphaeroides 2.4.1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q3IVH6 (Residues: 1-244; Coverage: 100%)
Gene name: RSP_4006
Sequence domains: Enoyl-(Acyl carrier protein) reductase
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 66.526Å b: 87.999Å c: 93.463Å
α: 90° β: 111.68° γ: 90°
R-values:
R R work R free
0.172 0.17 0.212
Expression system: Escherichia coli BL21(DE3)