3uxp

X-ray diffraction
2.72Å resolution

Co-crystal Structure of Rat DNA polymerase beta Mutator I260Q: Enzyme-DNA-ddTTP

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 38.4 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06766 (Residues: 1-335; Coverage: 100%)
Gene name: Polb
Sequence domains:
Structure domains:
DNA 5'-D(P*AP*TP*GP*TP*GP*AP*G)-3' Chains: D, P
Molecule details ›
Chains: D, P
Length: 7 nucleotides
Theoretical weight: 2.18 KDa
Source organism: Rattus norvegicus
Expression system: Not provided
DNA 5'-D(P*AP*CP*TP*CP*AP*CP*AP*TP*A)-3' Chains: E, T
Molecule details ›
Chains: E, T
Length: 9 nucleotides
Theoretical weight: 2.68 KDa
Source organism: Rattus norvegicus
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P21
Unit cell:
a: 100.321Å b: 56.607Å c: 93.144Å
α: 90° β: 102.04° γ: 90°
R-values:
R R work R free
0.24 0.218 0.292
Expression systems:
  • Escherichia coli
  • Not provided