› 3uxn

X-ray diffraction
2.5Å resolution

Crystal Structure of Rat DNA Polymerase Beta, Wild Type Apoenzyme

Released:
DOI: 10.2210/pdb3uxn/pdb
PDB 3uxn coloured by chain and viewed from the front.
PDB 3uxn coloured by chain and viewed from the side.
PDB 3uxn coloured by chain and viewed from the top.
Source organism: Rattus norvegicus
Primary publication:
Structural changes in the hydrophobic hinge region adversely affect the activity and fidelity of the I260Q mutator DNA polymerase β.
Gridley CL, Rangarajan S, Firbank S, Dalal S, Sweasy JB, Jaeger J
Biochemistry 52 4422-32 (2013)
PMID: 23651085

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase beta Chains: A, B
Molecule details ›
Chains: A, B
Length: 335 amino acids
Theoretical weight: 38.39 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P06766 (Residues: 1-335; Coverage: 100%)
Gene name: Polb
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P21
Unit cell:
a: 77.946Å b: 67.447Å c: 83.357Å
α: 90° β: 116.14° γ: 90°
R-values:
R R work R free
0.252 0.25 0.317
Expression system: Escherichia coli

Quick links

Citations

4 citation in other articles

Structure and mechanism of DNA polymerase β.
Beard et al. (2014)
3 more

1 mention without citation

Transitions in DNA polymerase β μs-ms dynamics related to substrate binding and catalysis.
DeRose et al. (2018)