3uwt

X-ray diffraction
2.5Å resolution

Crystal structure of a RNA binding domain of poly-U binding splicing factor 60KDa (PUF60) from Homo sapiens at 2.50 A resolution

Released:
Source organism: Homo sapiens
Entry authors: Joint Center for Structural Genomics (JCSG), Partnership for T-Cell Biology (TCELL)

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(U)-binding-splicing factor PUF60 Chain: A
Molecule details ›
Chain: A
Length: 200 amino acids
Theoretical weight: 22.28 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHX1 (Residues: 118-316; Coverage: 36%)
Gene names: FIR, PUF60, ROBPI, SIAHBP1
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P3121
Unit cell:
a: 64.173Å b: 64.173Å c: 80.337Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.202 0.24
Expression system: Escherichia coli