X-ray diffraction
1.6Å resolution

Ec_IspH in complex with buta-2,3-dienyl diphosphate (1300)


Function and Biology Details

Reaction catalysed:
Isopentenyl diphosphate + 2 oxidized ferredoxin [iron-sulfur] cluster + H(2)O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + 2 reduced ferredoxin [iron-sulfur] cluster + 2 H(+)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
4-hydroxy-3-methylbut-2-enyl diphosphate reductase Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 35.79 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
  • Canonical: P62623 (Residues: 1-316; Coverage: 100%)
Gene names: JW0027, b0029, ispH, lytB, yaaE
Sequence domains: LytB protein
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 70.4Å b: 80.51Å c: 111.73Å
α: 90° β: 90° γ: 90°
R R work R free
0.17 0.16 0.21
Expression system: Escherichia coli