› 3uub

X-ray diffraction
2.9Å resolution

The GLIC pentameric Ligand-Gated Ion Channel Loop2-21' mutant reduced in solution

Released:
DOI: 10.2210/pdb3uub/pdb
PDB 3uub coloured by chain and viewed from the front.
PDB 3uub coloured by chain and viewed from the side.
PDB 3uub coloured by chain and viewed from the top.
Source organism: Gloeobacter violaceus
Entry authors: Sauguet L, Nury H, Corringer PJ, Delarue M

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo pentamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Proton-gated ion channel Chains: A, B, C, D, E, F, G, H, I, J
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J
Length: 321 amino acids
Theoretical weight: 36.52 KDa
Source organism: Gloeobacter violaceus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q7NDN8 (Residues: 44-359; Coverage: 100%)
Gene names: glr4197, glvI
Sequence domains: Neurotransmitter-gated ion-channel ligand binding domain
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand PLC 30 x PLC
Ligand LMT 12 x LMT
Ligand NA 2 x NA
Ligand CL 10 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2
Unit cell:
a: 182.13Å b: 133.49Å c: 319.69Å
α: 90° β: 102.64° γ: 90°
R-values:
R R work R free
0.218 0.218 0.221
Expression system: Escherichia coli

Quick links

Citations

21 review citations

Druggable Lipid Binding Sites in Pentameric Ligand-Gated Ion Channels and Transient Receptor Potential Channels.
Cheng et al. (2021)
20 more

1 mention without citation

Structural basis for ion permeation mechanism in pentameric ligand-gated ion channels.
Sauguet et al. (2013)