Structure analysis

Crystal Structure of Nucleosome Core Particle Assembled with the 146b Alpha-Satellite Sequence (NCP146b)

X-ray diffraction
2.2Å resolution
Source organism: Xenopus laevis
Assembly composition:
hetero decamer (preferred)
Entry contents: 4 distinct polypeptide molecules
1 distinct DNA molecule

Assemblies

Assembly 1 (preferred)
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Multimeric state: hetero decamer
Accessible surface area: 71756.76 Å2
Buried surface area: 61134.8 Å2
Dissociation area: 4,587.13 Å2
Dissociation energy (ΔGdiss): 64.38 kcal/mol
Dissociation entropy (TΔSdiss): 14.39 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-117008

Macromolecules

Chains: A, E
Length: 135 amino acids
Theoretical weight: 15.3 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P84233 (Residues: 2-136; Coverage: 99%)
Pfam: Core histone H2A/H2B/H3/H4
InterPro:
CATH: Histone, subunit A

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Chains: B, F
Length: 102 amino acids
Theoretical weight: 11.26 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P62799 (Residues: 2-103; Coverage: 99%)
Pfam: Centromere kinetochore component CENP-T histone fold
InterPro:
CATH: Histone, subunit A

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Chains: C, G
Length: 129 amino acids
Theoretical weight: 13.98 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P06897 (Residues: 2-130; Coverage: 99%)
Pfam:
InterPro:
CATH: Histone, subunit A

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Chains: D, H
Length: 125 amino acids
Theoretical weight: 13.85 KDa
Source organism: Xenopus laevis
Expression system: Escherichia coli
UniProt:
  • Canonical: P02281 (Residues: 2-126; Coverage: 99%)
Pfam: Core histone H2A/H2B/H3/H4
InterPro:
CATH: Histone, subunit A

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Name: 146-mer DNA
Representative chains: I, J
Length: 146 nucleotides
Theoretical weight: 45.05 KDa

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