PDBe 3us5

X-ray diffraction
1.38Å resolution

Crystal structure of a RNA-binding domain of a poly-U binding splicing factor 60KDa (PUF60) from Homo sapiens at 1.38 A resolution

Released:
Source organism: Homo sapiens
Entry authors: Joint Center for Structural Genomics (JCSG), Partnership for T-Cell Biology (TCELL)

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Poly(U)-binding-splicing factor PUF60 Chain: A
Molecule details ›
Chain: A
Length: 118 amino acids
Theoretical weight: 13.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UHX1 (Residues: 443-559; Coverage: 21%)
Gene names: FIR, PUF60, ROBPI, SIAHBP1
Structure domains: Alpha-Beta Plaits

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P212121
Unit cell:
a: 43.433Å b: 48.974Å c: 56.298Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.144 0.142 0.181
Expression system: Escherichia coli