› 3urm

X-ray diffraction
1.8Å resolution

Crystal structure of the periplasmic sugar binding protein ChvE

Released:
DOI: 10.2210/pdb3urm/pdb
PDB 3urm coloured by chain and viewed from the front.
PDB 3urm coloured by chain and viewed from the side.
PDB 3urm coloured by chain and viewed from the top.
Source organism: Agrobacterium fabrum str. C58
Primary publication:
Agrobacterium tumefaciens recognizes its host environment using ChvE to bind diverse plant sugars as virulence signals.
Hu X, Zhao J, DeGrado WF, Binns AN
Proc. Natl. Acad. Sci. U.S.A. 110 678-83 (2013)
PMID: 23267119

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Multiple sugar-binding periplasmic receptor ChvE Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 35.87 KDa
Source organism: Agrobacterium fabrum str. C58
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25548 (Residues: 25-354; Coverage: 100%)
Gene names: AGR_C_4267, Atu2348, chvE
Sequence domains: Periplasmic binding protein domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand GAL 2 x GAL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P65
Unit cell:
a: 130.076Å b: 130.076Å c: 64.676Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.151 0.149 0.195
Expression system: Escherichia coli BL21(DE3)

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Citations

7 review citations

The role of solute binding proteins in signal transduction.
Matilla et al. (2021)
6 more

1 mention without citation

Agrobacterium tumefaciens recognizes its host environment using ChvE to bind diverse plant sugars as virulence signals.
Hu et al. (2013)