3up8

X-ray diffraction
1.96Å resolution

Crystal structure of a putative 2,5-diketo-D-gluconic acid reductase B

Released:
Source organism: Sinorhizobium meliloti 1021
Entry authors: Eswaramoorthy S, Chamala S, Evans B, Foti R, Gizzi A, Hillerich B, Kar A, LaFleur J, Seidel R, Villigas G, Zencheck W, Almo SC, Swaminathan S, New York Structural Genomics Research Consortium (NYSGRC)

Function and Biology Details

Reaction catalysed:
2-dehydro-D-gluconate + NADP(+) = 2,5-didehydro-D-gluconate + NADPH
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldo_ket_red domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 298 amino acids
Theoretical weight: 33.51 KDa
Source organism: Sinorhizobium meliloti 1021
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92RC6 (Residues: 1-275; Coverage: 100%)
Gene names: SMc00101, dkgB
Sequence domains: Aldo/keto reductase family
Structure domains: NADP-dependent oxidoreductase domain

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: P212121
Unit cell:
a: 57.283Å b: 88.768Å c: 123.638Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.237 0.211 0.24
Expression system: Escherichia coli