› 3up3

X-ray diffraction
1.25Å resolution

Nuclear receptor DAF-12 from hookworm Ancylostoma ceylanicum in complex with (25S)-cholestenoic acid

Released:
DOI: 10.2210/pdb3up3/pdb
PDB 3up3 coloured by chain and viewed from the front.
PDB 3up3 coloured by chain and viewed from the side.
PDB 3up3 coloured by chain and viewed from the top.
Source organisms:
Entry authors: Zhi X, Zhou XE, Melcher K, Motola DL, Gelmedin V, Hawdon J, Kliewer SA, Mangelsdorf DJ, Xu HE

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
NR LBD domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 243 amino acids
Theoretical weight: 27.77 KDa
Source organism: Ancylostoma ceylanicum
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: H0USY8 (Residues: 1-243; Coverage: 100%)
Gene name: daf-12
Sequence domains: Ligand-binding domain of nuclear hormone receptor
Structure domains: Retinoid X Receptor
Nuclear receptor coactivator 2 Chain: P
Molecule details ›
Chain: P
Length: 14 amino acids
Theoretical weight: 1.68 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q15596 (Residues: 741-754; Coverage: 1%)
Gene names: BHLHE75, NCOA2, SRC2, TIF2

Ligands and Environments

2 bound ligands:
Ligand XCA 1 x XCA
Ligand EDO 6 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 34.545Å b: 85.296Å c: 46.325Å
α: 90° β: 106.87° γ: 90°
R-values:
R R work R free
0.167 0.166 0.18
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

5 review citations

Helminth lipidomics: Technical aspects and future prospects.
Wang et al. (2021)
4 more

2 mentions without citation

Structural conservation of ligand binding reveals a bile acid-like signaling pathway in nematodes.
Zhi et al. (2012)
1 more